Fourteen athletes represented the United States in Australia at the 2025 UCI Masters Mountain Bike World Championships. Full results can be found here for Cross Country and Downhill.
The Team USA roster is as follows:
Cross Country
| Name | Hometown | Age Group | Place |
| WEIMAR Ashlee | Mead, WA | F40-44 | 6 |
| WRONSKA Beata | Brevard, NC | F50-54 | 4 |
| URBAN Cameron | Kentfield, CA | F55-59 | 1 |
| WAGNER Kimberly | Tempe, AZ | F60-64 | 3 |
| PFLUKE Lillian | F65-69 | 2 | |
| SCHROCK Sheri | Cohasset, MN | F70-74 | 1 |
| BRADFORD-PARISH Kevin | Mead, WA | M40-44 | 3 |
| PETERSON Cody | Bend, OR | M45-49 | 4 |
| JUAREZ David (Tinker) | Whittier, CA | M60-64 | 1 |
| HAYLES Thomas | Aspen, CO | M65-69 | 4 |
Downhill
| Name | Hometown | Age Group | Place |
| PFLUKE Lillian | F65-69 | 2 | |
| MELTON David | Chattanooga, TN | M65-69 | 6 |
| THOMPSON Rob | M60-64 | 8 |
